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Protein Molarity Formula:
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Protein molarity refers to the concentration of a protein solution expressed in moles per liter (M). It represents the number of protein molecules present in a given volume of solution and is essential for various biochemical and molecular biology applications.
The calculator uses the protein molarity formula:
Where:
Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of the protein and appropriate unit conversions.
Details: Accurate protein molarity calculation is crucial for experimental design, enzyme kinetics studies, protein-protein interaction assays, and ensuring proper stoichiometry in biochemical reactions.
Tips: Enter protein concentration in mg/mL and molecular weight in g/mol. Both values must be positive numbers. The calculator will provide molarity in M (mol/L).
Q1: Why convert mg/mL to molarity?
A: Molarity provides information about the number of molecules, which is essential for stoichiometric calculations and understanding molecular interactions.
Q2: How do I determine protein molecular weight?
A: Molecular weight can be calculated from amino acid sequence, measured by mass spectrometry, or estimated from SDS-PAGE migration.
Q3: What if my concentration is in μg/mL?
A: Convert μg/mL to mg/mL by dividing by 1000, then use the calculator with the converted value.
Q4: Are there limitations to this calculation?
A: This assumes the protein is pure and monomeric. For oligomeric proteins, use the molecular weight of the functional unit.
Q5: Why multiply by 1000 in the formula?
A: The factor 1000 converts mg to μg, ensuring proper unit alignment in the molarity calculation (g/mol requires mass in grams).